2026,Vol. 43(1): 26
研究报告
1 ,2-二氯乙烷降解菌YN5的筛选鉴定与土壤修复
杨宗政1,2,3， 王 浪1， 唐立娜1， 辛宗翰2， 吴 毅2， 吴志国1,2,3
1. 天津科技大学 海洋与环境学院， 天津 300457； 2. 天津科技大学 化工与材料学院，
天津 300457； 3. 天津市卤水化工与资源生态化利用重点实验室， 天津 300457
Screening, identification, and soil remediation of a 1 ,2-dichloroethane degrading bacterium YN5
YANG Zongzheng1,2,3, WANG Lang1, TANG Li’na1, XIN Zonghan 2, WU Yi 2, WU Zhiguo1,2,3
1. College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin 300457,
China; 2. College of Chemical Engineering and Materials Science, Tianjin University of Science and Technology,
Tianjin 300457, China; 3. Tianjin Key Laboratory of Brine Chemical Engineering and Resource Eco-utilization,
Tianjin 300457, China
摘要： 从某化工厂的1,2-二氯乙烷（1,2-dichloroethane，1,2-DCA）污染土壤中筛选得到1株能够高效降解1,2-DCA的细菌YN5，通过形态观察、生理生化测试和16S rRNA序列分析，初步鉴定该菌株为屈曲杆菌属（Ancylobactersp.）。通过单因素实验考察菌株YN5的生长和降解特性，并将其应用于污染土壤修复中。实验结果表明，菌株YN5在pH
7~10、NaCl质量浓度1~10 g/L和温度20~40 ℃的条件下均能有效降解1,2-DCA。在最适条件（温度30 ℃、pH
9、NaCl质量浓度1 g/L）下，YN5可在16 h内将200 mg/L的1,2-DCA降解完全。在实验室模拟条件下，YN5对130
mg/kg 1,2-DCA污染土壤的2
d去除率达到77.10%。研究发现，土壤含水率和环境温度对菌株YN5的修复效果具有明显影响。此外，YN5修复后的土壤酶活性显著改善，其中对脲酶活性提升最为明显，可将脲酶活性提高130.83%。菌株YN5具有高效降解1,2-DCA的能力，在1,2-DCA污染土壤修复中展现出潜在的应用前景。
关键词： 1,2-二氯乙烷,
Ancylobacter,
生物修复,
土壤修复,
土壤酶活
Abstract: In this paper, a
bacterium YN5 capable of degrading 1,2-DCA was screened from soil
contaminated with 1,2-dichloroethane (1,2-DCA) in a chemical plant.
Through morphological observation, physiological and biochemical tests,
and 16S rRNA sequence analysis, the strain was preliminarily identified
asAncylobactersp.. The growth and degradation characteristics of strain YN5 were
investigated by single factor experiment, and the strain was applied to
the remediation of contaminated soil. The experimental results showed
that strain YN5 could degrade 1,2-DCA effectively under the conditions
of pH 7－10, NaCl mass concentration 1－10 g/L, and temperature 20－40 ℃.
Under the optimal condition (temperature 30 ℃, pH 9, NaCl mass
concentration 1 g/L), YN5 could completely degrade 200 mg/L of 1,2-DCA
within 16 h. Under laboratory simulation conditions, the 2-day removal
rate of YN5 on 130 mg/kg 1,2-DCA contaminated soil reached 77.10%. It
was found that soil moisture content and ambient temperature
significantly affected the remediation effect of strain YN5. In
addition, the soil enzyme activity after YN5 restoration was
significantly improved, with the urease activity showing the most
obvious improvement, increasing by 130.83%. Strain YN5 had the ability
to efficiently degrade 1,2-DCA efficiently, showing potential
application prospects in the remediation of 1,2-DCA contaminated soil.
Key words: 1,2-dichloroethane,
Ancylobacter,
bioremediation,
soil remediation,
soil enzyme activity
收稿日期:
基金资助: VCM工厂含氯有机物污染土壤修复技术的研发及应用项目（2023120021000787）
作者简介: 杨宗政，博士，教授，研究方向为污染土壤修复，E-mail：yzz320@tust.edu.cn