2026,Vol. 43(1): 32
研究报告
蜡样芽孢杆菌合成并负载纳米金颗粒协同降解萘
曾现程1,2， 王梓丞1,2， 杜国标1， 沈 莺1,2， 龚玉华1,2， 武 超1,3， 邓国志1
1. 安徽大学 资源与环境工程学院， 合肥 230000； 2. 安徽省矿山生态修复工程实验室， 合肥 230000；
3. 安徽大学 生命科学学院， 合肥 230000
Synthesis and loading of gold nanoparticles by Bacillus cereus for synergistic degradation of naphthalene
ZENG Xiancheng1,2, WANG Zicheng1,2, DU Guobiao1, SHEN Ying1,2, GONG Yuhua1,2,WU Chao1,3, DENG Guozhi1
1. College of Resources and Environmental Engineering, Anhui University, Hefei 230000, China;
2. Anhui Province Engineering Laboratory for Mine Ecological Remediation, Hefei 230000, China;
3. School of Life Sciences, Anhui University, Hefei 230000, China
摘要： 本研究针对多环芳烃（polycyclic aromatic hydrocarbons， PAHs）污染问题，从东北大庆石油污染土筛选并鉴定了一株高效降解萘的细菌。通过形态观察、生理生化特性分析及16S rDNA基因序列同源性比较，确定该菌株为Bacillus cereusXC-6。实验结果表明，该菌株对多种苯系物具有较好的降解效果，菌株XC-6在30 ℃条件下120 h完全降解1000 mg/L萘、50
mg/L苊、50
mg/L二苯并呋喃、80%芴、30%二苯并噻吩以及40%菲，同时采用XC-6在有氧条件下合成并负载纳米金颗粒进行萘的协同降解，使其较单菌降解速率提升20%，96
h实现完全降解。通过优化培养条件，发现该菌株在适宜的温度、pH和接种量条件下，降解效率进一步提高。本研究为多环芳烃污染土壤的生物修复提供了理论依据和菌种资源，具有一定的应用前景。同时，为进一步研究该菌株负载纳米材料的降解机制和扩大其在环境治理中的应用奠定了基础。
关键词： Bacillus cereusXC-6,
萘,
纳米金颗粒,
生物降解,
生物杂合体
Abstract: To address polycyclic
aromatic hydrocarbons （PAHs） pollution, a naphthalene-degrading bacteria
was screened and identified from oil-contaminated soil in Daqing,
northern China. Through morphological observation,
physiological/biochemical characterization, and 16S rDNA sequence
analysis, the strain was identified asBacillus cereusXC-6. The results showed that the strain XC-6 could degrade 1000 mg/L
naphthalene, 50 mg/L acenaphthene, 50 mg/L dibenzofuran, 80% fluorene,
30% dibenzothiophene, and 50% phenanthrene within 120 h at 30 ℃. When
XC-6 was combined with gold nanoparticles synthesized under aerobic
conditions, the naphthalene degradation rate increased by 20% compared
to that of bacterial treatment alone, reaching complete degradation
within 96 h. By optimizing the culture conditions, it was found that the
degradation efficiency of the strain was further improved under
suitable temperature, pH and oxygen conditions. This study provided
theoretical basis and bacterial resources for the bioremediation of PAH
contaminated soil, demonstrating promising application potential.
Meanwhile, it established a foundation for investigating
nanomaterial-assisted degradation mechanisms and expanding its
applications in environmental applications.
Key words: Bacillus cereusXC-6,
naphthalene,
gold nanoparticles,
biodegradation,
biohybrid
收稿日期:
基金资助: 安徽高校自然科学重点研究项目（2023AH050107）
作者简介: 曾现程，硕士研究生，研究方向为污染控制与资源化，E-mail：905085936@qq.com